KERAGAMAN GENETIK BULU BABI TRIPNEUTES GRATILLA (LINNAEUS, 1758) DI PERAIRAN PANTAI DESA LAMBAKARA, SUMBA TIMUR, NUSA TENGGARA TIMUR (NTT)
DOI:
https://doi.org/10.58487/akrabjuara.v6i4.2061Keywords:
Genetic Diversity, Sea urchin Tripneutes gratillaAbstract
A total of 14 samples were taken from the coastal waters of East Sumba. The sample was isolated from genomic DNA using the KIT GENE AID method using GO TAQ Green PCR Mix material and universal primer pairs LCO1490 and HCO2198 to determine the expression of the amplified COI gene (PCR). The PCR results were sent to First Base CO (Malaysia) for sequencing. The sequence length of the COI gene sequencing of the genus Tripneustes from NTT waters using universal primers LCO1490 and HCO2198 was 690-700 bp. The results showed that the genetic diversity of the sea urchin population in the coastal waters of Sumba was 0.83516 while the value of nucleotide diversity was 0.00516. The total genetic distance ranged from 0.000-0.010. All population genetic distances are still relatively low because genetic distances less than 0.010-0.099 are included in the low category. This close genetic distance causes connectivity between the two populations because there is gene flow. Based on the phylogenetic analysis, it was found that the populations in Sumba waters and some areas (outgroups) were genetically close
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References
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Bazhin, A.G., Creaser, E.P., Barnes,
D.K.A., Botsford, L.W., Bradbury, A.,
Campbell, A., Dixon, J.D., Einarsson,
S., Gerring, P.K., Hebert, K., Hunter,
M., Hur, S.B., Johnson, C.R., JuinioMenez, M.A., Kalvass, P., Miller, R.J., Moreno, C.A., Palleiro, J.S., Rivas, D.,
Robinson, S.M.L., Schroeter, S.C.,
Steneck, R.S., Vadas, R.L., Woodby,
D.A., Xiaoqi, Z., 2002. Status and
management of world sea urchin
fisheries. In: Gibson, R.N., Barnes, M.,
Atkinson, R.J.A. (Eds.), Oceanography
and Marine Biology, 40, pp. 343–425.
Agatsuma, Sakai Y, Tajima K. 2010. Recent
advances in sea urchin aquaculture in
Japan. Bull. Aquacul. Assoc. Can. 108:
4–9.
Almany GR, SR Connolly, DD Heath, JD
Hogan, GP Jones, LJ McCook, M Mills,
RL Pressey & DH Williamson. 2009.
Connectivity, biodiversity conservation
and the design of marine reserve
networks for coral reefs. Coral Reefs,
28: 339–351
Ambarita MTD. 2003. Pengaruh kapur sirih
terhadap penanganan Bulu babi dan
kualitas gonad Bulu babi Tripneustes
gratilla (LINNAEUS) dan Echinothrix
calamaris (PALLAS). Jurnal Ilmu dan
Teknologi Pangan 1(1):94-105.
Andrew KNL, Agatsuma Y, Ballesteros E,
Bazhin AG, Creaser EP, Barnes DKA,
Botsford LW, Bradbury A., Campbell
A, Dixon JD, Einarsson S, Gerring P,
Bebert K, Hunter M, Hur SB, Johnson
CR, Juinio-Menez MA, Kalvass P,
Miller RJ, Moreno CA, Palleiro JS,
Rivas D, Robinson SML, Schroeter SC,
Steneck RS, Vadas RL, Woodby DA,
Xiaoqi Z. 2002. Status and management
of world sea urchin fisheries.
Oceanography and Marine Biology
Annual Review 40: 343–425.
Avise JC, Bowen BW, Lamp T. 1989. DNA
fingerprint from hypervariable
mitochondrial genotypes. Moleculer
Biologi Evolution, 6:258-269.
Avise JC. 1994. Molecular Marker, Natural
History and Evolution. Chapman and
Hall. United Stated America. Frankham,
R., Briscoe, D.A., & Ballou, J.D.
(2002). Introduction to conservation
genetics. 4th Ed. Cambridge Unikversity
Press. New York, 617 pp.
Barker MF. 2010. Recent advances in sea
urchin aquaculture in New Zealand and
Australia. Bull. Aquacul. Assoc. Can.
108: 10–17.
Benham, C. E., Supernault, K. J., & Burton,
R. S. (2012). Genetic assessment of the
population connectivity of the red urchin
(Strongylocentrotus franciscanus).
Journal of Experimental Marine Biology
and Ecology, 432–433, 47–54.
https://doi.org/10.1016/j.jembe.2012.07.
011
Chenoweth, S. 1994. The green sea urchin in
Maine — fishery and biology. Maine
Department of Marine Resources, W.
Boothbay Harbor, Maine. 10p.
Çiftci, Y. and Okumu?, ?. (2002) ‘Fish
population genetics and applications of
molecular markers to fisheries and
aquaculture: I-basic principles of fish
population genetics’, Turkish Journal of
Fisheries and Aquatic Sciences, 2(2002),
pp. 145–155.
Crivello, J. F., D. F. Danila, E. Lorda, M.
Keser & E. F. Roseman 2004. The
genetic stock structure of larval andjuvenile winter flounder
(Pseudopleuronectes americanus) larvae
in Connecticut waters of east- ern Long
Island Sound and estimations of larval
entrainment. J. Fish Res. 64:1–15.
Cowen, R.K. 2002. Larval dispersal and
retention and consequences for
population connectivity. Pp. 149–170 in
Coral Reef Fishes. Dynamics and
Diversity in a Complex Ecosystem. P.F.
Sale, ed., Academic Press, San Diego,
CA.
Daud SK, Mohammadi M, Siraj SS, Zakaria
MP. 2005. Morphometric Analysis of
Malaysian Oxudercine Goby,
Boleophthalmus boddarti (Pallas, 1770).
Pertanika Journal Tropical Agriculture
Science, 28 (2): 121-134.
Edgar G.J. 2000. Australian Marine Life: The
Plants and Animals of Temperate
Waters. New Holand, Sydney. 528pp.
Effendie, M. I. 2003. Telaah Kualitas Air Bagi
Pengelolaan Sumber Daya dan
Lingkungan Perairan. Kanisius,
Yogyakarta. hal 258.
Faterree, J., 2000. Marko alga dan Sisa sisa
hewan yang Mati Sebagai Makanan
Bulubabi Tripneustes gratilla. Balai
Penilitian Biologi Laut LON – LIPI.
Jakarta.
Frankham, R., Briscoe, D.A., & Ballou, J.D.
(2002). Introduction to conservation
genetics. 4th Ed. Cambridge Unikversity
Press. New York, 617 pp.
Frankham R 1999. Quantitative genetic in
conservation biology. Genetics Research
Committe, 74: 237-244.
Hagen NT, Siikavuopio SI. 2010. Recent
advances in sea urchin aquaculture in
Norway. Bull. Aquacul. Assoc. Can.
108: 18–22.
Hasan F. 2002. Pengaruh konsentrasi garam
terhadap mutu produk fermentasi gonad
Bulu babi jenis Tripneustes gratilla (L).
[skripsi]. Bogor (ID): Program Studi
Teknologi Hasil Perairan, Fakultas
Perikanan dan Ilmu Kelautan, Institut
Pertanian Bogor.
Hellberg ME, Burton RS, Neigel JE, &
Palumbi SR. 2002. Genetic assessment
of connectivity among marine
population. Bulletin of Marine Science,
70(1): 273–290.
Hughes AR, Inouye BD, Johnson MTJ,
Underwood N, Vellend M. 2008.
Ecological consequences of genetic
diversity. Ecology Letters, 11: 609-623.
Ihssen PE, Booke HE, Cas-Selman JM,
Mcglade JM, Payne NR, Utter FM.
1981. Stock identification : materials
and methods. Canadian Journal of
Fisheries and Aquatic Sciences, 38 (12):
1838-1855.
Jackson JBC. 1986. Modes of dispersal of
clonal benthic invertebrates:
consequences for species distribution
sand genetic structure of local
populations. Bull Mar Sci32: 588?606.
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Published
2022-12-13
How to Cite
Nomleni, A., Turnip, G., & Adin Afandi, L. (2022). KERAGAMAN GENETIK BULU BABI TRIPNEUTES GRATILLA (LINNAEUS, 1758) DI PERAIRAN PANTAI DESA LAMBAKARA, SUMBA TIMUR, NUSA TENGGARA TIMUR (NTT). Akrab Juara : Jurnal Ilmu-Ilmu Sosial, 6(4), 47–62. https://doi.org/10.58487/akrabjuara.v6i4.2061
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